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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
Cd45 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes <t>(CD45),</t> neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).
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Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes (CD45), neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).

Journal: iScience

Article Title: Targeted PGC-1α gene delivery by GV-assisted ultrasound cavitation for renal ischemia-reperfusion injury therapy

doi: 10.1016/j.isci.2025.114374

Figure Lengend Snippet: Ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs regulates EC functions (A and B) Western blot evaluation of the reversal of H/R or I/R-induced overexpression of adhesion molecules in ECs by ultrasound mediated gene transfection with Fuc-pPGC-1α-GVs in vitro and in vivo . Protein bands were quantitatively analyzed by a histogram. (C) Immunostaining of leukocytes (CD45), neutrophils (MPO), macrophages (F4/80), and T cells (CD3) in renal tissues from different groups on day 2 post renal I/R (scale bars: 50 μm). CD45/MPO/F4/80/CD3 + cells were quantitatively analyzed by histogram. (D) Representative images of the wound healing assay (scale bars: 200 μm). (E) The capillaries of renal tissues with or without ultrasound mediated Fuc-pPGC-1α-GVs cavitation were counted by immunofluorescence with CD31 on day 7 post renal I/R (scale bars: 100 μm). CD31 + cell was quantitatively analyzed by histogram. Data are expressed as the mean ± SD (A and B: n = 3, n represents the number of independent experiments; C and E: n = 3, n represents the number of mice in each group). t test was utilized for statistical analysis (∗∗ p < 0.01, ∗∗∗ p < 0.001).

Article Snippet: CD45 Antibody , Proteintech , Cat # 20103-1-AP; RRID: AB_2716813.

Techniques: Transfection, Western Blot, Over Expression, In Vitro, In Vivo, Immunostaining, Wound Healing Assay, Immunofluorescence